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Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow <t>cytometry</t> results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.
Flow Cytometry Staining Analysis, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow <t>cytometry</t> results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.
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Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow <t>cytometry</t> results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.
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Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow <t>cytometry</t> results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.
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Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow <t>cytometry</t> results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.
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Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow <t>cytometry</t> results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.
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Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow cytometry results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.

Journal: Journal of Inflammation Research

Article Title: Multi-Omics Analysis Identifies Immune Regulatory Networks in Sepsis-Associated Liver Injury: Experimental Validation and Clinical Relevance

doi: 10.2147/JIR.S515615

Figure Lengend Snippet: Immune markers were used to detect the type of immune cells in mice with sepsis and liver immune cell typing by single cell RNA sequencing. ( A ) Flow cytometry results of immune T lymphocytes (CD3+). ( B ) Flow cytometry results of immune B lymphocytes (CD19+). ( C ) Flow cytometry results of immune Macrophages (F4/80+). ( D ) Flow cytometry results of immune NK cells (NK1.1+). ( E ) Single-cell sequencing screened out the T-cell-related immune gene FCER1G in liver injury caused by sepsis. ( F ) Single-cell sequencing screened out the T-cell-related immune gene IL2RB in liver injury caused by sepsis. ( G ) Single-cell sequencing screened out the T-cell-related immune gene PTGDR in liver injury caused by sepsis. ( H ) Single-cell sequencing screened out the T-cell-related immune gene XCL1 in liver injury caused by sepsis.

Article Snippet: Flow Cytometry Staining & Analysis:T cells (Bioss Cat# bsm-30149A, PE, 1:150);B cells (Bioss Cat# bsm-30156A, FITC, 1:50);NK cells (Bioss Cat# bsm-30177M, PE, 1:100);Macrophages (Bioss Cat# bsm-30277A, PE, 1:50).

Techniques: RNA Sequencing, Flow Cytometry, Sequencing